![]() One of the primary differences between the three is the type of macromolecule they detect. These three blotting methods have similarities but different in a lot of things. ![]() There are different types of blotting procedures but the three common types are a northern blot, southern blot, and western blot. All three blotting techniques involve electrophoresis, transfer, and detection. (5, 8)Īutoradiograph, Detection of light, and Changes in colorīlotting is a special method used to identify a specific DNA, RNA, and protein from a given sample. What Knowledge Should Students Retain About Blotting Techniques Western blotting, Southern blotting, and Northern blotting are all techniques used to identify a particular type of macromolecule within a sample. My name is Ken and I’m an MCAT expert with MedSchoolCoach. Welcome back to another MCAT Mnemonic Monday. Used as a definite measure for Lyme disease, herpes, hepatitis B, and Creutzfeldt-Jacob disease. Ken Tao 9,825 1 minute read Ken Tao is the MedSchoolCoach expert on MCAT, and helps you remember Southern blot, Northern blot, and Western blot with the acronym: Snow Drop.It is useful in gene expression studies.Identify the specific RNA sequence in a given sample.To detect a specific DNA sequence in a given sample.It is used in paternity testing.It is used in DNA fingerprinting.It is used for victim identification.It is used for identifying criminals.It is useful in genetic diseases and infectious agents.It is used in mutation and identification of gene rearrangement. George Stark’s group at Stanford University in 1979 James Alwine, David Kemp, and George Stark in 1977 (9)Ī blotting procedure used for identifying a specific amino acid sequence in proteins. ![]() It is an adaptation of the southern blot procedure, which is useful in detecting a specific sequence of DNA through hybridization with complementary DNA. Point of comparisonĪ procedure used to identify a specific sequence of DNA. Image 12: The image shows the basic comparison between southern blot, northern blot, and western blot.
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